|
| Abstract No.: |
|
Scheduled at:
|
Friday, June 12, 2020, Hall D 9:20 AM
Medical Industry
|
|
| Title: |
In-vitro behavior of as-sprayed and post coating heat treated reinforced hydroxyapatite coatings deposited on SS316L by vacuum plasma spray technique
|
|
| Authors: |
Amardeep Singh Kang* / Lovely Professional University, India
Chander Prakash / Lovely Professional University, India
|
|
| Abstract: |
In this study the critical in-vitro examination of bare substrate, as-sprayed and heat treated reinforced HA coatings has been performed to assess the biocompatible behaviour of these bio-ceramic coatings. Briefly adherent fibroblast cell lines NIN3T3 and non-adherent human peripheral blood monocyte cells (hPBMCs) were used to assess the surface biocompatibility using standard MTT, trypan blue assays while their reactive oxygen species profiling was done to understand the potential stress that may hamper the human cell functioning. The results indicated that the as-sprayed and heat treated coatings exhibited excellent cell viability for hPBMCs and NIN3T3 cells in comparison to bare substrates. Similar results were observed for trypan blue dye exclusion assay. Furthermore, oxygen free radical levels indicated that there is little or no change in reactive oxygen species (ROS) levels in cells cultured on heat treated coatings. This implies that the materials used in coatings are not cytotoxic in nature and the phenomenon was true both for cell lines as well as human primary cells. Overall biological evaluation indicated that coatings are safe to human cells in primary conditions.
|
|