Direct laser interference patterning (DLIP) is a versatile laser technology which allows fabricating microstructures with high flexibility and therefore designing surface properties on a large diversity of materials, including metals, polymers, ceramics and coatings. In DLIP the main laser beam is divided in two or more sub-beams which are coherently overlapped on the surface of the material, where interference patterns in the micro and sub-micro range are created. By varying the processing conditions, patterns with different structure depth, lateral dimension and geometry can be produced. Suspension spraying has been investigated for the manufacture of coatings for different applications, such as thermal barrier coatings, photocatalysis or super-hydrophobicity. Even though these coatings are already extremely promising, tailored coatings are nowadays mandatory to reach the desired functionality or to improve the lifetime of the coating system. In this work DLIP technology is applied to modify the surface topography of suspension sprayed TiO2 coatings. In particular, a nanosecond-pulsed laser has been employed to produce line-like micro-structures with spatial periods between 3 µm and 7 µm and depths ranging from 200 nm up to 10 µm. The resulting surface patterns are characterized by white-light interferometry, scanning electron microscopy and Raman spectroscopy and the influence of the surface topography on the wettability behavior and photocatalytic activity has been investigated.