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4632 |
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Thursday, May 12, 2016, Room 3E 2:00 PM Medical Industry |
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CeO2 incorporated calcium silicate coatings protect
MC3T3-E1 osteoblastic cells from H2O2-induced oxidative stress
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Kai Li* / Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai Li Kai / Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Xie Youtao/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Huang Liping/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Zheng Xuebin/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China |
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Oxidative stress regulates cellular functions in multiple pathological conditions, including bone formation by osteoblastic cells. In this work, the protective effects of CeO2 incorporated calcium silicate coatings (CeO2-CS) on the response of osteoblast to H2O2-induced oxidative stress and the related mechanism were examined. CeO2 incorporation significantly increased osteoblast growth and reduced cell apoptosis caused by H2O2 when compared to control. H2O2-induced reduction of differentiation marker ALP was recovered in the presence of CeO2. The above effects were mediated by the antioxidant effect of CeO2. CeO2-CS coatings immersed in 1 mM H2O2 aqueous solution were able to degrade 90% of it in 1 week. In addition, CeO2 incorporation decreased reactive oxygen species production, suppressed malondialdehyde (MDA) information and reversed the reduced superoxide dismutase activity in H2O2-treated osteoblasts. Taken together, CeO2-CS coatings with antioxidant property would be promising for bone regeneration. |