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4633 |
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Thursday, May 12, 2016, Room 3E 2:20 PM Medical Industry |
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Synergy effects of Sr and Si ions from Sr-doped calcium silicate coatings on stimulation of osteogenesis |
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Kai Li / Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai Dandan Hu* / Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Li Kai/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Xie Youtao/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Huang Liping/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China Zheng Xuebin/ Shanghai Institute of Ceramics, Chinese Academy of Sciences, China |
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Sr has been reported to be able to stimulate osteogenesis, which is critical for bone generation. Silicate ceramics and coatings have also been reported to have stimulatory effects on osteogenesis due to the Si ion release. Therefore, we hypothesize that a combination of Sr and Si ions may show synergy effects on osteogenesis. In this work, ±-CaSiO3 (CS) and 5% Sr-±-CaSiO3 (Sr-CS) coatings were synthesized to identify the effect of Sr and Si ions on the osteogenic ability. The cytocompatibility of the coatings was assessed by testing the influence of ion extracts on proliferation of MC3T3-E1 cells. Thereafter, gene expressions of osteoblast differentiation makers in ion extracts were evaluated and mineralized nodules formation was observed. Results revealed that, as compared to CS extracts and the media containing soluble SrCl2, Sr-CS extracts possessed stronger stimulatory effects on up-regulation of osteoblast differentiation markers and mineralized nodules formation. Lower concentrations of Sr and Si ions released from Sr-CS than those released from pure CS or SrCl2 were enough to stimulate osteogenesis, which indicated that there were synergy effects between Sr and Si ions during stimulation of osteogenesis by Sr-CS. Taken together, the designed Sr-CS coating may be suitable as a new biomaterial for bone generation in.orthopedic application. |